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I3268
Sigma
Interleukin-6 human
solution, from human (osteosarcoma cells), cell culture tested
| Synonym: | IL-6 |
| MDL number: | MFCD00131403 |
Description
| Biochem/physiol Actions | Interleukin-6 is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. |
| Preparation Note | Produced in MG-63 osteosarcoma cells induced with human IL-1β.1 |
| Unit Definition | One unit equals the amount of IL-6 required to induce half maximal proliferation of T-1165 cell in vitro. |
| Physical form | Solution in 0.2 μm filtered phosphate buffered saline containing 0.1% human serum albumin. |
Properties
| biological source | from human (osteosarcoma cells) |
| assay | ≥90% (SDS-PAGE) |
| form | solution |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | B |
| WGK Germany | 3 |
References
| Cited Reference | 1. Van Damme, J., et al., Interleukin 1 and poly(rI).poly(rC) induce production of a hybridoma growth factor by human fibroblasts. Eur. J. Immunol. 17, 1-7, (1987) Abstract |
| reference | Hirano, T. The Cytokine Handbook 3rd ed.,, (1998), 197 |
| Gauldie, J., et al., Interferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc. Natl. Acad. Sci. U. S. A. 84, 7251, (1987) | |
| Van Snick, J., Interleukin-6: an overview. Annu. Rev. Immunol. 1990, 253 | |
| Van Snick, J., Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. Proc. Natl. Acad. Sci. U. S. A. 83, 9679, (1986) | |
| Nordan, R., A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science 233, 566, (1986) |
